His-Tag Rabbit pAb - #T0051
(7)
(3)
| 产品: | His-Tag 兔 pAb |
| 货号: | T0051 |
| 描述: | Rabbit polyclonal antibody to His-Tag pAb |
| 应用: | WB |
| 反应: | All |
| 蛋白号: | |
| RRID: | AB_2837584 |
产品描述
来源:
Rabbit
应用:
WB 1:3000-1:10000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
All
克隆:
Polyclonal
特异性:
The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
RRID:
AB_2837584
引用格式: Affinity Biosciences Cat# T0051, RRID:AB_2837584.
引用格式: Affinity Biosciences Cat# T0051, RRID:AB_2837584.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
抗原和靶标
免疫原:
Rabbit polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
描述:
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
文献引用
1). Lipopolysaccharide-binding protein expression is increased by stress and inhibits monoamine synthesis to promote depressive symptoms. Immunity, 2023
(PubMed: 36854305)
[IF=32.4]
2). A PD-1 peptide antagonist exhibits potent anti-tumor and immune regulatory activity. CANCER LETTERS, 2020
(PubMed: 32805322)
[IF=9.7]
3). The 1316T> C missenses mutation in MTHFR contributes to MTHFR deficiency by targeting MTHFR to proteasome degradation. Aging-US, 2021
(PubMed: 33290257)
[IF=5.2]
4). Characterization of a dual-CRD galectin in the silkworm Bombyx mori. Developmental & Comparative Immunology, 2016
(PubMed: 26944801)
[IF=2.9]
Application: WB Species: Sample:
5). A single-CRD C-type lectin is important for bacterial clearance in the silkworm. Developmental & Comparative Immunology, 2016
(PubMed: 27519466)
[IF=2.9]
6). Expression and characterization of recombinant humanized anti-HER2 single-chain antibody in Pichia pastoris for targeted cancer therapy. BIOTECHNOLOGY LETTERS, 2015
(PubMed: 25735855)
[IF=2.7]
Application: IF/ICC Species: Sample:
7). Molecular cloning and analysis of a C-type lectin from silkworm Bombyx mori. Archives of Insect Biochemistry and Physiology, 2017
(PubMed: 28618068)
[IF=2.2]
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