Caspase 1 Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IHC
Cites

1/70

Western blot analysis of Caspase 1 expression in 293 cells.

AF5418 at 1/100 staining Mouse spleen tissue by IHC-P.

AF5418 at 1/100 staining Mouse lung tissue by IHC-P.

AF5418 at 1/100 staining Rat spleen tissue by IHC-P.

$Fig.2 |NaAsO2 induced autophagy, activation of NLRP3 inflammasome and hepatic stellate cell in vivo. (A) The expression level of LC3 and p62 in rat liver following exposure to NaAsO2. The protein fraction was analyzed by Western blot. (B)The expression level of cytoplasmic CTSB in rat livers following exposure to NaAsO2.The protein fraction was analyzed by Western blot. (C) The expression level of NLRP3 inflammasome-associated proteins in rat livers following exposure to NaAsO2.The protein fraction was analyzed by Western blot.$

Fig.

$Fig.2 NaAsO2 induced autophagy, activation of NLRP3 inflammasome and 662 hepatic stellate cell in vivo. (A) The expression level of LC3 and p62 in rat livers 663 following exposure to NaAsO2. The protein fraction was analyzed by Western blot. (B) 664 The expression level of cytoplasmic CTSB in rat livers following exposure to NaAsO2. 665 The protein fraction was analyzed by Western blot. (C) The expression level of NLRP3 inflammasome-associated proteins in rat livers following exposure to NaAsO2. 667 The protein fraction was analyzed by Western blot. (D) The expression level of 668 Collagen- , α-SMA, MMP-1, and TIMP-1in rat livers following exposure to NaAsO2. 669 The protein fraction was analyzed by Western blot. (E) α-SMA was detected by IF. 670 White arrows indicate positive staining. Values are mean ± SD, and n = 5. *p < 0.05, 671 **p < 0.01 compared with the control group; Scale bar = 100 µm$

Fig.

$Fig.3 NaAsO2 impairs insulin signaling via activation of NLRP3 inflammasome in HepG2 cells. HepG2 cells were pretreated with 1 μg/ml LPS for 4 hours, 5 μM MCC950, for 4 hours, and were then treated with 4 μM NaAsO2 for 24 hours. Cytosolic fractions were analyzed by Western blot analysis. GAPDH was used as an internal control. The efficiency of MCC950, and its effect on NLRP3, caspase-1 activation, IL-1β and IL- 18 production in NaAsO2-treated HepG2 cells (A-E). HepG2 cells were stimulated with 100 nM insulin for 10 min at the end of treatment. Cytosolic fractions were analyzed by Western blot analysis. GAPDH was used as an internal control. The efficiency of MCC950, and its effect on p-IRS (Ser 307)/IRS1 and p-AKT (Ser473)/AKT1 ratio in NaAsO2-treated HepG2 cells (F-H). Insulin‐ stimulated glucose uptake in HepG2 cells was measured using a glucose assay kit (I). Results are mean ± SEM (n = 3). *P < 0.05 compare with the LPS group. #P<0.05 compare with 4 μM NaAsO2 group.$

Fig.

Figure 4.

Fig.

FIGURE 8| Effect of calycosin on the expression of proteins associated with the AMPK/SKP2/CARM1 signalling pathway in TNF-αinduced C2C12 cells in vitro.

Fig.

Figure 5 The expression levels of p-P38, P38, p-IκBα, caspase 1, COX2, and iNOS proteins in AD + NS, AD + donepezil, and AD + BM25 groups.

Fig.

FIGURE 9 Effects of OCOP and COP on the NLRP3 inflammasome in DSS-induced mice (A) Representative Western blotting images of NLRP3, ASC, Caspase-1and Caspase-1 p10 (B–E) The protein expression levels of NLRP3, ASC, Caspase-1 and Caspase-1 p10 in the NLRP3 inflammasome.

Figure 4 Effects of Rg1 on the expression levels of NLRP1, cleaved-caspase-1, IL-1β, NF-κB and p-NF-κB in LPS-induced HT22 cells determined by western blotting.

FIGURE 7 Effect of DHB on NLRP3 inflammasome signaling in pO/HX-induced hyperuricemic mice.

Figure 6: |MEL attenuated the protein levels of NLRP3, TLR4, NF-κB, and caspase-1 of the terminal ileum tissues in NEC mice.

Fig.

Figure 6.

Figure 6 |C-PC treatment suppresses the expression of NLRP3 inflammasome and NF-κB signaling pathway in lung tissues.

FIGURE 1 |Effects of combined administration of RvE1 and LXA4 on pro-inflammatory factor expression.

FIGURE s4 |The total proteins of infected primary peritoneal macrophages were extracted at 24, 36, 48, and 72 h post-infection and then subjected for Western blotting.

Figure 4.

Figure 6 Effect of HPC on the expression of caspase-1, GSDMD, and NLRP3 in the brains of cerebral I/R injury model mice.

Figure 7.

Figure 5.

Fig.

Figure 5 Effects of PAL and Col (1 µM) on NF-κB/NLRP3 and Nrf2 signaling pathways in LPS plus MSU-activated THP-1 cells.

Figure 3 (a) Immunofluorescent staining labeled pyroptosis marker caspase-1 was conducted to assess the extent of pyroptosis in each group of mice (scale bar = 50 μm); (b) Western blot was used to examine the expression of pyroptosis markers in the muscle homogenate of each group of mice.

Fig.

FIGURE 10 Effect of TTWC on the Level of NLRP3, ASC, pro-caspase-1, caspase-1, pro-IL-1beta, IL-1beta, pro-IL-18 and IL-18 protein in the prefrontal cortex (B1-B8) (Mean ± SD, n = 3).

Figure 5 Milrinone diminishes the secretion of inflammatory cytokines and inhibits the formation of NLRP3 inflammasomes.

Figure 6 Tuina treatment influenced the expression of pyroptosis-related molecules in the hippocampi of HIE rats.

Figure 4 Hypoxia induces fibroblast-like synoviocytes (FLS) pyroptosis by upregulating the expression of nucleotide-binding oligomerization segment-like receptor family 3 (NLRP3) inflammasome in FLS and the effect of monomeric derivatives of paeoniflorin (MDP).

Figure 4 Quercetin alleviated ethanol-induced pyroptosis via the nuclear transfer of PGC1α.

Figure 6 PSG suppressed pyroptosis of the liver tissues in IBD mice.

FIGURE 4 Caspase-1–dependent canonical pathway activated RTEC pyroptosis.

Figure 5.

Figure 9 The expression of caspase 1 and caspase 3 in lung tissues was detected by immunohistochemistry.

Figure 10 The expressions of cleaved caspase 1/caspase 1 and cleaved caspase 3/caspase 3 in lung tissues were detected by western blot.

Figure 4 mRNA and protein expression levels of NLRP3 inflammasome.

Figure 8.

Figure 5 Effects of SeNPs on the protein expression of jejunal Casepase-1.

Figure 3 Fig.

FIGURE 2 PTS reduces reduction of NLRP3 inflammasome and apoptosis in DOX-treated mice.

Figure 5 Effect of DF supplementation on the activation of NLRP3 inflammasome.

FIGURE 8 CGGE inhibited NLRP3 Inflammasome in ipsilateral cortex.

图 6 柴胡皂苷D对免疫性肝纤维化大鼠肝脏组织中NLRP3炎症小体和细胞焦亡蛋白的影响(n=8，x±s).

FIGURE 2 PTS reduces reduction of NLRP3 inflammasome and apoptosis in DOX-treated mice.

expression in lung cancer.

Figure 1 Expression of NLRP3, Caspase-1, and GSDMD in the decidua of pregnant rats in each group.

Fig.

FIGURE 2 Effects of AB4 treatment on inflammatory infiltration and protein levels of IL‐1β, GFAP, NLRP3 and caspase‐1 in the spinal cord of the control, CIA and CIA + AB4 groups.

FIGURE 2 PTS reduces reduction of NLRP3 inflammasome and apoptosis in DOX-treated mice.

Figure 5 Que induces GSDMD-mediated pyroptosis in colon cancer cells through NLRP3 inflammasome activation.

Figure 5 BA suppresses pyroptosis and promotes anti-oxidation in the spinal cord of SCI rats.

Figure 4 Effects of AST and CY-09 on NLRP3, caspase-1 expression in 661W and ARPE-19 cells A: The mRNA levels of NLRP3 and caspase-1 in 661W cells; B: The protein levels of NLRP3 and cleaved caspase-1 in 661W and ARPE-19 cells.

FIGURE 2 PTS reduces reduction of NLRP3 inflammasome and apoptosis in DOX-treated mice.

Fig.

产品:	Caspase 1 抗体
货号:	AF5418
描述:	Rabbit polyclonal antibody to Caspase 1
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Rabbit
分子量:	45kDa(Pro); 45kD(Calculated).
蛋白号:	P29466
RRID:	AB_2837902

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

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MS Report

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产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(89%), Rabbit(89%)

克隆:

Polyclonal

特异性:

Caspase 1 Antibody detects endogenous levels of total Caspase 1.

RRID:

AB_2837902
引用格式: Affinity Biosciences Cat# AF5418, RRID:AB_2837902.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

CASP-1; CASP1; CASP1_HUMAN; Caspase 1; Caspase-1 subunit p10; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1 beta converting enzyme; IL1B convertase; Interleukin 1 beta convertase; Interleukin 1B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45;

抗原和靶标

免疫原:

Uniprot:

P29466(CASP1_HUMAN) >>Visit HPA database.

基因/基因ID:

CASP1(834)

表达:

P29466 CASP1_HUMAN:

Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.

描述:

序列:

P29466 CASP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADKVLKEKRKLFIRSMGEGTINGLLDELLQTRVLNKEEMEKVKRENATVMDKTRALIDSVIPKGAQACQICITYICEEDSYLAGTLGLSADQTSGNYLNMQDSQGVLSSFPAPQAVQDNPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKDSVGVSGNLSLPTTEEFEDDAIKKAHIEKDFIAFCSSTPDNVSWRHPTMGSVFIGRLIEHMQEYACSCDVEEIFRKVRFSFEQPDGRAQMPTTERVTLTRCFYLFPGH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Bovine

Rabbit

Horse

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P29466 作为底物

P29466

Site	PTM Type	Source
S16	Phosphorylation	Uniprot
T21	Phosphorylation	Uniprot
T32	Phosphorylation	Uniprot
K37	Ubiquitination	Uniprot
K44	Ubiquitination	Uniprot
T49	Phosphorylation	Uniprot
K53	Ubiquitination	Uniprot
K134	Ubiquitination	Uniprot
K148	Ubiquitination	Uniprot
S149	Phosphorylation	Uniprot
K158	Ubiquitination	Uniprot
K204	Ubiquitination	Uniprot
T226	Phosphorylation	Uniprot
S227	Phosphorylation	Uniprot
K268	Ubiquitination	Uniprot
K274	Ubiquitination	Uniprot
K278	Ubiquitination	Uniprot
S306	Phosphorylation	Uniprot
K319	Ubiquitination	Uniprot
K320	Ubiquitination	Uniprot
K325	Ubiquitination	Uniprot
S376	Phosphorylation	Uniprot

研究背景

P29466_CASP1_HUMAN

功能:

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly.

翻译修饰:

The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

细胞定位:

Cytoplasm. Cell membrane.

组织特异性:

亚基结构:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p10 and p20 subunits interact with MEFV. Interacts with CARD17/INCA and CARD18. Interacts with SERPINB1; this interaction regulates CASP1 activity.

蛋白家族:

Belongs to the peptidase C14A family.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)

文献引用

1). Tetrahedral Framework Nucleic Acids Based Small Interfering RNA Targeting Receptor for Advanced Glycation End Products for Diabetic Complications Treatment. ACS Nano, 2023 (PubMed: 37751401) [IF=17.1]

2). Arsenic induces hepatic insulin resistance via mtROS-NLRP3 inflammasome pathway. JOURNAL OF HAZARDOUS MATERIALS, 2020 (PubMed: 32544768) [IF=13.6]

Application: WB Species: Human Sample: HepG2 cells

Fig.3 NaAsO2 impairs insulin signaling via activation of NLRP3 inflammasome in HepG2 cells. HepG2 cells were pretreated with 1 μg/ml LPS for 4 hours, 5 μM MCC950, for 4 hours, and were then treated with 4 μM NaAsO2 for 24 hours. Cytosolic fractions were analyzed by Western blot analysis. GAPDH was used as an internal control. The efficiency of MCC950, and its effect on NLRP3, caspase-1 activation, IL-1β and IL- 18 production in NaAsO2-treated HepG2 cells (A-E). HepG2 cells were stimulated with 100 nM insulin for 10 min at the end of treatment. Cytosolic fractions were analyzed by Western blot analysis. GAPDH was used as an internal control. The efficiency of MCC950, and its effect on p-IRS (Ser 307)/IRS1 and p-AKT (Ser473)/AKT1 ratio in NaAsO2-treated HepG2 cells (F-H). Insulin‐ stimulated glucose uptake in HepG2 cells was measured using a glucose assay kit (I). Results are mean ± SEM (n = 3). *P < 0.05 compare with the LPS group. #P<0.05 compare with 4 μM NaAsO2 group.

3). Water-extracted Lonicera japonica polysaccharide attenuates allergic rhinitis by regulating NLRP3-IL-17 signaling axis. Carbohydrate Polymers, 2022 (PubMed: 36184153) [IF=11.2]

4). Lactobacillus rhamnosus GG ameliorates triptolide-induced liver injury through modulation of the bile acid-FXR axis. Pharmacological research, 2024 (PubMed: 38908615) [IF=9.3]

5). Autophagic-CTSB-inflammasome axis modulates hepatic stellate cells activation in arsenic-induced liver fibrosis. Chemosphere, 2020 (PubMed: 31669990) [IF=8.8]

Application: WB Species: rat Sample: livers

Fig.2 |NaAsO2 induced autophagy, activation of NLRP3 inflammasome and hepatic stellate cell in vivo. (A) The expression level of LC3 and p62 in rat liver following exposure to NaAsO2. The protein fraction was analyzed by Western blot. (B)The expression level of cytoplasmic CTSB in rat livers following exposure to NaAsO2.The protein fraction was analyzed by Western blot. (C) The expression level of NLRP3 inflammasome-associated proteins in rat livers following exposure to NaAsO2.The protein fraction was analyzed by Western blot.

Application: WB Species: rat Sample: livers

Fig.2 NaAsO2 induced autophagy, activation of NLRP3 inflammasome and 662 hepatic stellate cell in vivo. (A) The expression level of LC3 and p62 in rat livers 663 following exposure to NaAsO2. The protein fraction was analyzed by Western blot. (B) 664 The expression level of cytoplasmic CTSB in rat livers following exposure to NaAsO2. 665 The protein fraction was analyzed by Western blot. (C) The expression level of NLRP3 inflammasome-associated proteins in rat livers following exposure to NaAsO2. 667 The protein fraction was analyzed by Western blot. (D) The expression level of 668 Collagen- , α-SMA, MMP-1, and TIMP-1in rat livers following exposure to NaAsO2. 669 The protein fraction was analyzed by Western blot. (E) α-SMA was detected by IF. 670 White arrows indicate positive staining. Values are mean ± SD, and n = 5. *p < 0.05, 671 **p < 0.01 compared with the control group; Scale bar = 100 µm

6). Combination of resolvin E1 and lipoxin A4 promotes the resolution of pulpitis by inhibiting NF-κB activation through upregulating sirtuin 7 in dental pulp fibroblasts. CELL PROLIFERATION, 2022 (PubMed: 35411569) [IF=8.5]

Application: WB Species: rat Sample: Dental pulp fibroblasts

FIGURE 1 |Effects of combined administration of RvE1 and LXA4 on pro-inflammatory factor expression.(C) NLRP3, (D) caspase-1, (E) IL-1β and (F) IL-18 mRNA levels on LPS-induced DPFs detected by qPCR and (G) their protein levels tested by western blotting (normalized to that of β-tubulin).

7). Gegen Qinlian pills alleviate carrageenan-induced thrombosis in mice model by regulating the HMGB1/NF-κB/NLRP3 signaling. PHYTOMEDICINE, 2022 (PubMed: 35413645) [IF=7.9]

8). Cinnamic acid preserves against myocardial ischemia/reperfusion injury via suppression of NLRP3/Caspase-1/GSDMD signaling pathway. Phytomedicine, 2022 (PubMed: 35320770) [IF=7.9]

9). Cardioprotective activity of ethyl acetate extract of Cinnamomi Ramulus against myocardial ischemia/reperfusion injury in rats via inhibiting NLRP3 inflammasome activation and pyroptosis. Phytomedicine, 2021 (PubMed: 34673348) [IF=7.9]

10). Iguratimod ameliorates bleomycin-induced pulmonary fibrosis by inhibiting the EMT process and NLRP3 inflammasome activation. Biomedicine & Pharmacotherapy, 2022 (PubMed: 36076570) [IF=7.5]

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Caspase 1 Antibody - #AF5418

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产品描述

抗原和靶标

种属预测

翻译修饰 - P29466 作为底物

研究背景

研究领域

文献引用

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