VCAM1 Antibody - #DF6082

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产品: VCAM1 抗体
货号: DF6082
描述: Rabbit polyclonal antibody to VCAM1
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
分子量: 80~120kDa; 81kD(Calculated).
蛋白号: P19320
RRID: AB_2838050

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(82%), Bovine(%), Horse(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
VCAM1 Antibody detects endogenous levels of total VCAM1.
RRID:
AB_2838050
引用格式: Affinity Biosciences Cat# DF6082, RRID:AB_2838050.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD106; CD106 Antigen; INCAM 100; INCAM-100; L1CAM; MGC99561; V-CAM 1; Vascular Cell Adhesion Molecule 1; Vascular cell adhesion protein 1; VCAM 1; VCAM-1; VCAM1; VCAM1_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P19320(VCAM1_HUMAN) >>Visit HPA database.

基因/基因ID:
VCAM1(7412)
表达:
P19320 VCAM1_HUMAN:

Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

描述:
Vascular cell adhesion protein 1 (VCAM1) is important in cell-cell recognition. It has been reported to function in leukocyte-endothelial cell adhesion, interact with the beta-1 integrin VLA4 on leukocytes, and mediate both adhesion and signal transduction. There are two isoforms; Isoform 1, a single-pass type I membrane protein, and isoform 2, a lipid-anchor (GPI-anchor). VCAM1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. It is also expressed in the bone marrow (1).
序列:
MPGKMVVILGASNILWIMFAASQAFKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPELLVLYFASSLIIPAIGMIIYFARKANMKGSYSLVEAQKSKV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Rabbit
91
Pig
82
Bovine
82
Dog
82
Chicken
70
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

翻译修饰:

Sialoglycoprotein.

细胞定位:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

蛋白家族:

Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.

研究领域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). A novel UV-curable extravascular stent to prevent restenosis of venous grafts. Composites Part B: Engineering, 2021 [IF=12.7]

Application: WB    Species: Rat    Sample:

Fig. 6. A: PCNA immunofluorescence staining of venous graft vessels in rats. B, C: Western blot analysis of α-SMA, PCNA, ICAM-1, VCAM-1, and vimentin in rat vein graft vessels. D: ICAM-1 immunofluorescence staining of venous graft vessels in rats. E: qPCR of α-SMA, PCNA, ICAM-1, VCAM-1, and vimentin in rat vein graft vessels. Data are presented as the mean ± SD; *: compared with control group, p < 0.05. #: compared with sham group,
2). Novel Strategy for Isolation of Mice Bone Marrow–Derived Endothelial Cells (BMECs). Stem Cell Research & Therapy, 2020 (PubMed: 33941266) [IF=7.5]

Application: WB    Species: mouse    Sample: primary bone marrow endothelial cells

Fig. 4 |Characterization of primary bone marrow endothelial cells by RT-QPCR and immunoblottinge .The immunoblotting analysis of primary bone marrow endothelial after 7 days of cultured further substantiates the purity of endothelial cells with protein ladder (M) (N= 3 indicates independent experiments for RT-qPCR and N=2 for immunoblotting with six mice/group(12 femora and 12 tibias). *p<0.05 **p<0.01, and ***p<0.001

Application: WB    Species: Mice    Sample: bMECs

Fig. 4 Characterization of primary bone marrow endothelial cells by RT-QPCR and immunoblotting: a–d the relative mRNA expression of primary bone marrow endothelial cells compared to CD31 microbead-negative selected cells. The bar chart indicated the fold of the gene expression of primary BMECs compared to the CD31 microbead-negative selected cells. The fold change is quantified by the Pitfall method, followed by a Student’s test comparison between the CD31 microbead-negative selected cells and endothelial cells. P value ≤0.05 considered being statistically significant. e The immunoblotting analysis of primary bone marrow endothelial after 7 days of cultured further substantiates the purity of endothelial cells with protein ladder (M) (N= 3 indicates independent experiments for RT-qPCR and N=2 for immunoblotting with six mice/group (12 femora and 12 tibias). *p<0.05 **p<0.01, and ***p<0.001
3). Traditional Chinese Medicine Shi-Bi-Man ameliorates psoriasis via inhibiting IL-23/Th17 axis and CXCL16-mediated endothelial activation. Chinese medicine, 2024 (PubMed: 38429819) [IF=5.3]
4). Activation of vascular endothelial cells by synovial fibrosis promotes Netrin-1-induced sensory nerve sprouting and exacerbates pain sensitivity. Journal of cellular and molecular medicine, 2023 (PubMed: 37702437) [IF=5.3]
5). NAG-1/GDF15 inhibits diabetic nephropathy via inhibiting AGE/RAGE-mediated inflammation signaling pathways in C57BL/6 mice and HK-2 cells. Life Sciences, 2022 (PubMed: 36367498) [IF=5.2]
6). Nepeta angustifolia attenuates responses to vascular inflammation in high glucose-induced human umbilical vein endothelial cells through heme oxygenase-1 induction. JOURNAL OF ETHNOPHARMACOLOGY, 2019 (PubMed: 30419276) [IF=4.8]
7). Sanhuang Xiexin decoction ameliorates DSS-induced colitis in mice by regulating intestinal inflammation, intestinal barrier, and intestinal flora. JOURNAL OF ETHNOPHARMACOLOGY, 2022 (PubMed: 35843414) [IF=4.8]
8). Novel therapeutic targets for atherosclerosis: Targeting the FOSB-MECP2-Commd1 pathway. International immunopharmacology, 2025 (PubMed: 39566383) [IF=4.8]
9). FGF18 alleviates sepsis-induced acute lung injury by inhibiting the NF-κB pathway. Respiratory research, 2024 (PubMed: 38419044) [IF=4.7]
10). New evidence for T-cadherin in COVID-19 pathogenesis, endothelial dysfunction, and lung fibrosis. Frontiers in cell and developmental biology, 2025 (PubMed: 40109358) [IF=4.6]
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