CBS Antibody - #DF6410

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产品: CBS 抗体
货号: DF6410
描述: Rabbit polyclonal antibody to CBS
应用: WB IHC
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Rabbit, Xenopus
分子量: 61kDa; 61kD(Calculated).
蛋白号: P35520
RRID: AB_2838373

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Sheep(100%), Rabbit(93%), Xenopus(86%)
克隆:
Polyclonal
特异性:
CBS Antibody detects endogenous levels of total CBS.
RRID:
AB_2838373
引用格式: Affinity Biosciences Cat# DF6410, RRID:AB_2838373.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AI047524; AI303044; Beta thionase; Beta-thionase; Cbs; Cbs cystathionine beta-synthase; CBS_HUMAN; Cystathionine beta synthase; Cystathionine beta-synthase; EC 4.2.1.22; HIP 4; HIP4; Methylcysteine synthase; MGC18856; MGC18895; MGC37300; OTTHUMP00000109416; OTTHUMP00000109418; Serine sulfhydrase;

抗原和靶标

免疫原:
Uniprot:

P35520(CBS_HUMAN) >>Visit HPA database.

基因/基因ID:
CBS(102724560)
表达:
P35520 CBS_HUMAN:

In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.

描述:
The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964). This antibody is specific to CBS.
序列:
MPSETPQAEVGPTGCPHRSGPHSAKGSLEKGSPEDKEAKEPLWIRPDAPSRCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVRINKIGKKFGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGTLKPGDTIIEPTSGNTGIGLALAAAVRGYRCIIVMPEKMSSEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSAGSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEEDLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVDEAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLSHILEMDHFALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERDQK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
93
Xenopus
86
Chicken
71
Pig
0
Horse
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P35520 作为底物

Site PTM Type Enzyme
T5 Phosphorylation
C15 S-Nitrosylation
S19 Phosphorylation
S27 Phosphorylation
S32 Phosphorylation
K72 Ubiquitination
K75 Ubiquitination
K82 Ubiquitination
K83 Ubiquitination
T87 Phosphorylation
K102 Ubiquitination
C109 S-Nitrosylation
K119 Ubiquitination
K137 Ubiquitination
K177 Ubiquitination
R190 Methylation
T191 Phosphorylation
T193 Phosphorylation
S199 Phosphorylation
K211 Sumoylation
K211 Ubiquitination
S227 Phosphorylation Q13976 (PRKG1)
S254 Phosphorylation
T257 Phosphorylation
T262 Phosphorylation
K322 Ubiquitination
K325 Ubiquitination
R336 Methylation
K384 Ubiquitination
R389 Methylation
K394 Ubiquitination
K398 Sumoylation
K398 Ubiquitination
K481 Acetylation
K481 Ubiquitination
K485 Ubiquitination
S525 Phosphorylation Q13976 (PRKG1)

研究背景

功能:

Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity).

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.

亚基结构:

Homotetramer.

蛋白家族:

Belongs to the cysteine synthase/cystathionine beta-synthase family.

研究领域

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

文献引用

1). Total flavones of Rhododendron induce the transformation of A1/A2 astrocytes via promoting the release of CBS-produced H2S. PHYTOMEDICINE, 2023 (PubMed: 36701996) [IF=7.9]

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