产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0528, RRID:AB_2833796.
展开/折叠
5730450D16Rik; 6330400I15; ASTBDN; GA19; Gastric cancer antigen Ga19; MGC29428; mNAT1; N alpha acetyltransferase 15, NatA auxiliary subunit; N terminal acetyltransferase; N(alpha) acetyltransferase 15, NatA auxiliary subunit; N-alpha-acetyltransferase 15; N-terminal acetyltransferase; Naa15; NAA15_HUMAN; NatA auxiliary subunit; NATH; NMDA receptor regulated 1; NMDA receptor regulated protein 1; NMDA receptor-regulated protein 1; Protein tubedown 1; Protein tubedown-1; Tbdn 1; Tbdn100; Transcriptional coactivator tubedown 100; tubedown-1;
抗原和靶标
Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy.
- Q9BXJ9 NAA15_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPAVSLPPKENALFKRILRCYEHKQYRNGLKFCKQILSNPKFAEHGETLAMKGLTLNCLGKKEEAYELVRRGLRNDLKSHVCWHVYGLLQRSDKKYDEAIKCYRNALKWDKDNLQILRDLSLLQIQMRDLEGYRETRYQLLQLRPAQRASWIGYAIAYHLLEDYEMAAKILEEFRKTQQTSPDKVDYEYSELLLYQNQVLREAGLYREALEHLCTYEKQICDKLAVEETKGELLLQLCRLEDAADVYRGLQERNPENWAYYKGLEKALKPANMLERLKIYEEAWTKYPRGLVPRRLPLNFLSGEKFKECLDKFLRMNFSKGCPPVFNTLRSLYKDKEKVAIIEELVVGYETSLKSCRLFNPNDDGKEEPPTTLLWVQYYLAQHYDKIGQPSIALEYINTAIESTPTLIELFLVKAKIYKHAGNIKEAARWMDEAQALDTADRFINSKCAKYMLKANLIKEAEEMCSKFTREGTSAVENLNEMQCMWFQTECAQAYKAMNKFGEALKKCHEIERHFIEITDDQFDFHTYCMRKITLRSYVDLLKLEDVLRQHPFYFKAARIAIEIYLKLHDNPLTDENKEHEADTANMSDKELKKLRNKQRRAQKKAQIEEEKKNAEKEKQQRNQKKKKDDDDEEIGGPKEELIPEKLAKVETPLEEAIKFLTPLKNLVKNKIETHLFAFEIYFRKEKFLLMLQSVKRAFAIDSSHPWLHECMIRLFNTAVCESKDLSDTVRTVLKQEMNRLFGATNPKNFNETFLKRNSDSLPHRLSAAKMVYYLDPSSQKRAIELATTLDESLTNRNLQTCMEVLEALYDGSLGDCKEAAEIYRANCHKLFPYALAFMPPGYEEDMKITVNGDSSAEAEELANEI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BXJ9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Ubiquitination | Uniprot | |
| K34 | Acetylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K62 | Acetylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| Y66 | Phosphorylation | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| T180 | Phosphorylation | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| K262 | Acetylation | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| Y280 | Phosphorylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K419 | Ubiquitination | Uniprot | |
| K425 | Ubiquitination | Uniprot | |
| R442 | Methylation | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| K454 | Ubiquitination | Uniprot | |
| K459 | Ubiquitination | Uniprot | |
| K467 | Ubiquitination | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| Y538 | Phosphorylation | Uniprot | |
| K543 | Ubiquitination | Uniprot | |
| T584 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| K646 | Ubiquitination | Uniprot | |
| K649 | Ubiquitination | Uniprot | |
| K659 | Ubiquitination | Uniprot | |
| T662 | Phosphorylation | Uniprot | |
| K665 | Ubiquitination | Uniprot | |
| C721 | S-Nitrosylation | Uniprot | |
| K724 | Acetylation | Uniprot | |
| K724 | Ubiquitination | Uniprot | |
| K735 | Acetylation | Uniprot | |
| K735 | Ubiquitination | Uniprot | |
| K748 | Acetylation | Uniprot | |
| K756 | Acetylation | Uniprot | |
| K756 | Methylation | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| S759 | Phosphorylation | Uniprot | |
| K770 | Ubiquitination | Uniprot | |
| K781 | Ubiquitination | Uniprot | |
| T850 | Phosphorylation | Uniprot | |
| S855 | Phosphorylation | Uniprot | |
| S856 | Phosphorylation | Uniprot |
研究背景
Auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter.
Cleaved by caspases during apoptosis, resulting in a stable 35 kDa fragment.
Cytoplasm. Nucleus.
Note: Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes.
Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy.
Component of the N-terminal acetyltransferase A (NatA) complex composed of NAA10 or probably NAA11 and NAA15. Interacts with XRCC6, NAA50 and XRCC5. Associates with HYPK when in a complex with NAA10. Interaction with HYPK reduces the capacity to interact with NAA50.
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