(1)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6714, RRID:AB_2838676.
展开/折叠
AI528772; c-cpn; CCT alpha; CCT; CCT-alpha; CCT1; Ccta; CCTalpha; D6S230E; MGC133746; p63; T complex 1; T complex protein 1 alpha subunit; T complex protein 1; T-complex homolog TCP1; T-complex protein 1 subunit alpha; T-complex protein 1 subunit alpha B; Tailless complex polypeptide 1; Tailless complex polypeptide 1A; Tailless complex polypeptide 1B; TCP 1 alpha; Tcp-1; TCP-1-alpha; TCP1; TCPA_HUMAN; Tp63; TRic;
抗原和靶标
- P17987 TCPA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSSLGPVGLDKMLVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAELLKNADELVKQKIHPTSVISGYRLACKEAVRYINENLIVNTDELGRDCLINAAKTSMSSKIIGINGDFFANMVVDAVLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESMLISGYALNCVVGSQGMPKRIVNAKIACLDFSLQKTKMKLGVQVVITDPEKLDQIRQRESDITKERIQKILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSLHDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPNTLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTIVKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGALND
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P17987 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| R11 | Methylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| T13 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| C76 | S-Nitrosylation | Uniprot | |
| K84 | Acetylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| Y121 | Phosphorylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| C147 | S-Nitrosylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| Y181 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| R185 | Methylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| K199 | Acetylation | Uniprot | |
| K199 | Methylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| C236 | S-Nitrosylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| K259 | Acetylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| Y299 | Phosphorylation | Uniprot | |
| C357 | S-Nitrosylation | Uniprot | |
| K365 | Acetylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| C397 | S-Nitrosylation | Uniprot | |
| K400 | Acetylation | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| S429 | Phosphorylation | Uniprot | |
| R443 | Methylation | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| R468 | Methylation | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| K484 | Acetylation | Uniprot | |
| K484 | Ubiquitination | Uniprot | |
| S491 | Phosphorylation | Uniprot | |
| K494 | Ubiquitination | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| K510 | Ubiquitination | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| K532 | Acetylation | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| K538 | Acetylation | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| K541 | Acetylation | Uniprot | |
| K541 | Ubiquitination | Uniprot | |
| S544 | Phosphorylation | Uniprot | |
| Y545 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot |
研究背景
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Component of the chaperonin-containing T-complex (TRiC), a heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Interacts with GBA.
Belongs to the TCP-1 chaperonin family.
文献引用
Application: WB Species: Human Sample: OV-90 and SKOV-3 cell
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