NOX4 Antibody - #DF6924

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产品: NOX4 抗体
货号: DF6924
描述: Rabbit polyclonal antibody to NOX4
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 67kDa; 67kD(Calculated).
蛋白号: Q9NPH5
RRID: AB_2838883

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(82%)
克隆:
Polyclonal
特异性:
NOX4 Antibody detects endogenous levels of total NOX4.
RRID:
AB_2838883
引用格式: Affinity Biosciences Cat# DF6924, RRID:AB_2838883.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Kidney oxidase 1; Kidney oxidase-1; Kidney superoxide producing NADPH oxidase; Kidney superoxide-producing NADPH oxidase; KOX 1; KOX; Kox-1; KOX1; NADPH; NADPH oxidase 4; Nox4; NOX4_HUMAN; Renal NAD(P)H oxidase; Renal NAD(P)H-oxidase; RENOX;

抗原和靶标

免疫原:
Uniprot:

Q9NPH5(NOX4_HUMAN) >>Visit HPA database.

基因/基因ID:
NOX4(50507)
表达:
Q9NPH5 NOX4_HUMAN:

Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.

描述:
NOX4 (NADPH oxidase 4 ) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex.NOX4 has 7 isforms and the molecular weight are 7KDa,26-32KDa,58-67KDa,75-80KDa(PMID:11728818).This antibody is specific to NOX4.
序列:
MAVSWRSWLANEGVKHLCLFIWLSMNVLLFWKTFLLYNQGPEYHYLHQMLGLGLCLSRASASVLNLNCSLILLPMCRTLLAYLRGSQKVPSRRTRRLLDKSRTFHITCGVTICIFSGVHVAAHLVNALNFSVNYSEDFVELNAARYRDEDPRKLLFTTVPGLTGVCMVVVLFLMITASTYAIRVSNYDIFWYTHNLFFVFYMLLTLHVSGGLLKYQTNLDTHPPGCISLNRTSSQNISLPEYFSEHFHEPFPEGFSKPAEFTQHKFVKICMEEPRFQANFPQTWLWISGPLCLYCAERLYRYIRSNKPVTIISVMSHPSDVMEIRMVKENFKARPGQYITLHCPSVSALENHPFTLTMCPTETKATFGVHLKIVGDWTERFRDLLLPPSSQDSEILPFIQSRNYPKLYIDGPFGSPFEESLNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLRRLYFIWVCRDIQSFRWFADLLCMLHNKFWQENRPDYVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGKTVGVFCCGPNSLSKTLHKLSNQNNSYGTRFEYNKESFS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
91
Chicken
82
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9NPH5 作为底物

Site PTM Type Enzyme
Y491 Phosphorylation
Y566 Phosphorylation

研究背景

功能:

Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.

Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1.

翻译修饰:

Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.

细胞定位:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cell junction>Focal adhesion.
Note: May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.

Nucleus. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.

亚基结构:

Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4.

文献引用

1). Chronic infusion of ELABELA alleviates vascular remodeling in spontaneously hypertensive rats via anti-inflammatory, anti-oxidative and anti-proliferative effects. ACTA PHARMACOLOGICA SINICA, 2022 (PubMed: 35260820) [IF=8.2]
2). Human umbilical cord mesenchymal stem cells alleviate chemotherapy-induced premature ovarian insufficiency mouse model by suppressing ferritinophagy-mediated ferroptosis in granulosa cells. Free radical biology & medicine, 2024 (PubMed: 38677487) [IF=7.4]
3). Protective Role of Dioscin against Doxorubicin-Induced Chronic Cardiotoxicity: Insights from Nrf2-GPX4 Axis-Mediated Cardiac Ferroptosis. Biomolecules, 2024 (PubMed: 38672439) [IF=5.5]

Application: WB    Species: Rat    Sample:

Figure 8 Dioscin suppresses cardiac ferroptosis by regulating the Nrf2/GPX4 signaling pathway in DOX-induced rats. (A) Representative Western blotting images of Nrf2, HO-1, and TfR1 in DOX-induced rat treated with or without dioscin. (B–D) Bar charts indicate the protein expression levels of Nrf2, HO-1, and TfR1 in cardiac tissues. Data are mean ± SD (n = 3). (E–K) Bar charts indicate the mRNA expression levels of Nrf2, HO-1, TfR1, DMT1, FTL, FTH1, and FPN in cardiac tissues. Data are mean ± SD (n = 6). (L) Representative Western blotting images of NOX4, ABCB8, and FXN in DOX-induced rat treated with or without dioscin. (M–O) Bar charts indicate the protein expression levels of NOX4, ABCB8, and FXN in cardiac tissues. Data are mean ± SD (n = 3). (P–R) Bar charts indicate the mRNA expression levels of NOX4, ABCB8, and FXN in cardiac tissues. Data are mean ± SD (n = 6). (S) Representative images of immunofluorescence analysis of Nrf2 and NOX4 expression were captured by fluorescence microscopy. (T,U) The bar chart indicates the Nrf2 and NOX4 intensity in cardiac tissue. Data are mean ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. DOX treatment group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. control group. (A,L) Original western blotting figures can be found in Figures S5 and S6.
4). Caviunin glycoside (CAFG) from Dalbergia sissoo attenuates osteoarthritis by modulating chondrogenic and matrix regulating proteins. Journal of Ethnopharmacology, 2022 (PubMed: 34116187) [IF=5.4]
5). Geranylgeranylacetone, an inducer of heat shock protein 70, attenuates pulmonary fibrosis via inhibiting NF-κB/NOX4/ROS signalling pathway in vitro and in vivo. Chemico-Biological Interactions, 2023 (PubMed: 37307957) [IF=5.1]
6). Enhancer of zeste homolog 2 modulates oxidative stress-mediated pyroptosis in vitro and in a mouse kidney ischemia-reperfusion injury model. FASEB JOURNAL, 2020 (PubMed: 31914694) [IF=4.8]

Application: WB    Species: mouse    Sample: kidneys

FIGURE 6|EZH2 inhibition alleviated pyroptosis through Nox4. A, Representative bands of western blot analysis for the expression of Nox4 and bar graphs showed the fold changes, n = 3.
7). Inhibition of (Pro)renin Receptor-Mediated Oxidative Stress Alleviates Doxorubicin-Induced Heart Failure. Frontiers in Oncology, 2022 (PubMed: 35574363) [IF=4.7]

Application: WB    Species: Rat    Sample: H9C2 cells

Figure 3 PRR inhibition downregulated the overexpression of oxidant species and apoptosis in H9C2 cells treated with doxorubicin. (A) Cell viability of H9C2 cells treated with Doxorubicin (DOX) treated at different concentrations (0–10 μM). (B) The protein expression of PRR downregulated by PRR-siRNA in H9C2 cells. (B1) Quantification of PRR/GAPDH protein expression levels relative to changing PRR expression. (C) Representative oxidative stress based on DHE relative fluorescence intensity of H9C2 cells. (D) Flow cytometry shows that PRR expression is directly related to cell apoptosis in doxorubicin (DOX)-treated H9C2 cells. (E) The protein expression of RAC1, NOX2, NOX4, and cleaved caspase3 was regulated by PRR inhibition in H9C2 cells. (E1)-(E4) Quantification of RAC1/GAPDH, NOX2/GAPDH, NOX4/GAPDH, and cleaved caspase3/GAPDH protein expression levels relative to changing PRR expression. (*p < 0.05, **p < 0.01 vs the control group. # p < 0.05, ## p < 0.01vs DOX group).
8). Protective effect of Berberine on reproductive function and spermatogenesis in diabetic rats via inhibition of ROS/JAK2/NFκB pathway. Andrology, 2020 (PubMed: 32012485) [IF=4.5]

Application: WB    Species: rat    Sample: testis

FIGURE 3|BB attenuates ROS production in the testis of DM rats. (A) Representative Western blot results for NOX5, p22phox, gp91phox, NOX1, NOX3, and NOX4 in the testes of rats from all four groups.
9). Ebselen ameliorates renal ischemia–reperfusion injury via enhancing autophagy in rats. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2022 (PubMed: 35338455) [IF=4.3]
10). Eupatilin attenuates doxorubicin-induced cardiotoxicity by activating the PI3K-AKT signaling pathway in mice. Molecular and Cellular Biochemistry, 2023 (PubMed: 37222879) [IF=4.3]
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