产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0568, RRID:AB_2834193.
展开/折叠
Cardiac lineage protein 1; CLP 1; CLP1; EDG 1; EDG1; Estrogen down-regulated gene 1 protein; FLJ13562; Hexamethylene bis acetamide inducible 1; Hexamethylene bis acetamide inducible protein; Hexamethylene bis acetamide inducible transcript 1; Hexamethylene bis-acetamide-inducible protein 1; HEXI1_HUMAN; HEXIM 1; Hexim1; HEXIM1 protein; HIS 1; HIS1; HMBA inducible; MAQ 1; MAQ1; Menage a quatre 1; Menage a quatre protein 1; Protein HEXIM1;
抗原和靶标
Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
- O94992 HEXI1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEPFLSEYQHQPQTSNCTGAAAVQEELNPERPPGAEERVPEEDSRWQSRAFPQLGGRPGPEGEGSLESQPPPLQTQACPESSCLREGEKGQNGDDSSAGGDFPPPAEVEPTPEAELLAQPCHDSEASKLGAPAAGGEEEWGQQQRQLGKKKHRRRPSKKKRHWKPYYKLTWEEKKKFDEKQSLRASRIRAEMFAKGQPVAPYNTTQFLMDDHDQEEPDLKTGLYSKRAAAKSDDTSDDDFMEEGGEEDGGSDGMGGDGSEFLQRDFSETYERYHTESLQNMSKQELIKEYLELEKCLSRMEDENNRLRLESKRLGGDDARVRELELELDRLRAENLQLLTENELHRQQERAPLSKFGD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O94992 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S66 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| R146 | Methylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| S158 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K165 | Acetylation | Uniprot | |
| Y167 | Phosphorylation | Uniprot | |
| Y168 | Phosphorylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| T206 | Phosphorylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| T270 | Phosphorylation | Uniprot | |
| Y271 | Phosphorylation | Uniprot | |
| R273 | Methylation | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| K284 | Acetylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K289 | Acetylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| Y291 | Phosphorylation | Uniprot | |
| K296 | Acetylation | Uniprot | |
| K296 | Methylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| R331 | Methylation | Uniprot | |
| T341 | Phosphorylation | Uniprot | |
| R347 | Methylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| K356 | Ubiquitination | Uniprot |
研究背景
Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Nucleus. Cytoplasm.
Note: Binds alpha-importin and is mostly nuclear (PubMed:16362050).
Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Interacts with CCNT2; mediates formation of a tripartite complex with KPNA2. Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 non-coding RNA.
The coiled-coil domain mediates oligomerization.
Belongs to the HEXIM family.
限制条款
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