产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0272, RRID:AB_2833353.
展开/折叠
BS4; NEDD8 ultimate buster 1; Negative regulator of ubiquitin like proteins 1; Negative regulator of ubiquitin-like proteins 1; NUB1; NUB1_HUMAN; NUB1L; NY REN 18; NYREN18; Renal carcinoma antigen NY-REN-18;
抗原和靶标
Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.
- Q9Y5A7 NUB1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQKKYLQAKLTQFLREDRIQLWKPPYTDENKKVGLALKDLAKQYSDRLECCENEVEKVIEEIRCKAIERGTGNDNYRTTGIATIEVFLPPRLKKDRKNLLETRLHITGRELRSKIAETFGLQENYIKIVINKKQLQLGKTLEEQGVAHNVKAMVLELKQSEEDARKNFQLEEEEQNEAKLKEKQIQRTKRGLEILAKRAAETVVDPEMTPYLDIANQTGRSIRIPPSERKALMLAMGYHEKGRAFLKRKEYGIALPCLLDADKYFCECCRELLDTVDNYAVLQLDIVWCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQGIRNYHSGNDVEAYEYLNKARQLFKELYIDPSKVDNLLQLGFTAQEARLGLRACDGNVDHAATHITNRREELAQIRKEEKEKKRRRLENIRFLKGMGYSTHAAQQVLHAASGNLDEALKILLSNPQMWWLNDSNPETDNRQESPSQENIDRLVYMGFDALVAEAALRVFRGNVQLAAQTLAHNGGSLPPELPLSPEDSLSPPATSPSDSAGTSSASTDEDMETEAVNEILEDIPEHEEDYLDSTLEDEEIIIAEYLSYVENRKSATKKN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y5A7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K10 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| T103 | Phosphorylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| Y126 | Phosphorylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K198 | Acetylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| Y212 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| Y239 | Phosphorylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| Y360 | Phosphorylation | Uniprot | |
| K365 | Ubiquitination | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| T569 | Phosphorylation | Uniprot |
研究背景
Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.
Nucleus.
Note: Predominantly nuclear.
Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.
Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. Isoform 1 binds to NEDD8 more efficiently than isoform 2. Interacts with AIPL1. The interaction with UBD via UBA domains facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates UBD degradation and that of its conjugates.
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